CRISPR and Gene Editing

Source-derived questions

Questions extracted from the papers in this topic, excluding the curated questions above.

• How do bacteria use CRISPR and Cas9 as a natural immune system against viruses?
• How do bacteria use CRISPR as an immune memory against viruses?
• How do base editing and prime editing correct single-letter DNA mutations without cutting both strands?
• How does CRISPR-Cas9 function as a bacterial immune system against viruses?
• How does a guide RNA let CRISPR-Cas9 find one exact sequence among three billion base pairs?
• How quickly did CRISPR-Cas9 spread across different organisms and applications after 2013?
• What controversy erupted at the end of 2015 over CRISPR's use in human embryos?
• What did Charpentier and Doudna's 2012 paper show could be done with the CRISPR-Cas9 system?
• What did Doudna and Charpentier show in 2012 that made CRISPR-Cas9 programmable?
• What did Michael Smith's site-directed mutagenesis technique let researchers do to a gene?
• What diseases have CRISPR-based therapies already reached clinical trials for?
• What fourteen genetic modifications were made to the donor pig before the March 2024 transplant into Richard Slayman?

Sources

• Breakthrough Prize in Life Sciences
• CRISPR gene editing named Breakthrough of the Year
• CRISPR-Cas9 demonstrated as a programmable genome editor in human cells
• First pig kidney transplanted into a living human recipient
• Nobel Prize in Chemistry
• Nobel Prize in Chemistry

Connected topics